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Checkpoint kinase 2 (CHK2) is multifunctional player in the DNA-damage response signalling pathway, especially at the S and G2 checkpoints of cell cycle. The protein consits of three functional domains. The T-terminal SQ/TQ cluster (SCD motif), the forkhead associated domain (FHA) and the kinase domain. Following DNA damage, ATM or ATR kinase phosphorylates CHK2 at the SCD motif. Thereafter dimerization and autophosphorylation takes place. Activated CHK2 phosphorylates e.g. Cdc25A, B, and C and E2F-1 and causes cell cycle arrest and apoptosis.
Affinity-purified recombinant human checkpoint homologue kinase (CHK2) expressed in insect cells featuring an affinity tag.
Highly active form of CHK2 suitable for labeling CHK substrates
Protein concentration (Bradford with BSA as standard) 0.4 mg/ml
Purity > 95% by SDS PAGE
Specific activity > 142,600 U*/mg
Molecular weight based on amino acid sequence 66 kDa
Entrez Gene ID: 11200
UniProtKB: O96017
Ordering information: shipped on dry ice
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Chaturvedi P, Eng WK, Zhou BB et al. (1999) "Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway" Oncogene 18(28):4047-54
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McGowan CH (2002) "Checking in on Cds1 (Chk2): A checkpoint kinase and tumor suppressor" Bioessays 24(6):502-11
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