Recombinant human CDK1/CycB, active protein kinase, 10 µg

Cdks are the catalytic subunits of serine/threonine protein kinases which are involved in the progression through the cell cycle. The catalytic activity of these kinases requires interaction with cyclins, which are synthesized and degraded during the cell cycle in a cyclical fashion. CDK1/CycB complexes regulate several events during both, the G2-M transition and mitosis. Active complexes undergo translocation to the nucleus and phosphorylate nuclear substrates, which are necessary for mitotic onset. Cytoplasmic CDK1/CycB is involved in regulatory and structural processes, such as chromosomal condensation and fragmentation of the Golgi network. The inactivation of CDK1/CycB complex is accomplished by degradation of cyclin B by anaphase-promoting complex, allowing mitotic exit and cell division.

Recombinant human cyclin-dependent protein kinase CDK1 (cdc2), human CDK1, amino acids M1 - M297, and human CycB1, amino acids M1-V433 both N -terminal GST-HIS6 fusion protein  with a Thrombin cleavage site, coexpressed in Sf9 insect cells

Theoretical MW GST-CDK1: 63.882 kDa
Theoretical MW GST-CycB1: 78.925 kDa
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.255 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 7,600 pmol/mg x min
Activation: This kinase was not activated by special procedures

Entrez Gene ID: 983/891  UniProtKB:  P06493/ P14635

Ordering information: shipped on dry ice

Product specific literature references:

Draetta G, Piwnica-Worms H, Morrison D, Druker B, Roberts T, Beach D (1988) "Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate." Nature 336(6201):738-44

Draetta G, Luca F, Westendorf J, Brizuela L, Ruderman J, Beach D (1989) "Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF." Cell 56(5):829-38

Brizuela L, Draetta G, Beach D (1989) "Activation of human CDC2 protein as a histone H1 kinase is associated with complex formation with the p62 subunit." Proc Natl Acad Sci U S A. 86(12):4362-6.

Solomon MJ, Glotzer M, Lee TH, Philippe M, Kirschner MW (1990) "Cyclin activation of p34cdc2." Cell. 63(5):1013-24

Lees JA, Buchkovich KJ, Marshak DR, Anderson CW, Harlow E (1991) "The retinoblastoma protein is phosphorylated on multiple sites by human cdc2." EMBO J. 10(13):4279-90

Dalton S (1992) "Cell cycle regulation of the human cdc2 gene." EMBO J. 11(5):1797-804

Desai D, Gu Y, Morgan DO (1992) "Activation of human cyclin-dependent kinases in vitro." Mol Biol Cell. 3(5):571-82

Datta NS, Williams JL, Caldwell J, Curry AM, Ashcraft EK, Long MW (1996) "Novel alterations in CDK1/cyclin B1 kinase complex formation occur during the acquisition of a polyploid DNA content" Mol Biol Cell 7(2):209-23

Meijer L, Borgne A, Mulner O, Chong JP, Blow JJ, Inagaki N, Inagaki M, Delcros JG, Moulinoux JP (1997) "Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5." Eur J Biochem. 243(1-2):527-36

Wheatley SP, Hinchcliffe EH, Glotzer M, Hyman AA, Sluder G, Wang Y (1997) "CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo." J Cell Biol. 138(2):385-93

Schultz C, Link A, Leost M, Zaharevitz DW, Gussio R, Sausville EA, Meijer L, Kunick C (1999) "Paullones, a series of cyclin-dependent kinase inhibitors: synthesis, evaluation of CDK1/cyclin B inhibition, and in vitro antitumor activity." J Med Chem. 42(15):2909-19

Takizawa CG, Morgan DO (2000) "Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C." Curr Opin Cell Biol. 12(6):658-65

Doree M, Hunt T (2002) "From Cdc2 to Cdk1: when did the cell cycle kinase join its cyclin partner?" J Cell Sci. 115(Pt 12):2461-4

Ubersax JA, Woodbury EL, Quang PN, Paraz M, Blethrow JD, Shah K, Shokat KM, Morgan DO (2003) "Targets of the cyclin-dependent kinase Cdk1" Nature 425 (6960):859-64