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Recombinant Biotinylated Human VEGF121, Avi Tag (Avitag™), 25µg  

Recombinant Biotinylated Human VEGF121, Avi Tag (Avitag™), 25µg

Recombinant Biotinylated Human VEGF121Protein, AA Ala 27 - Arg 147, expressed from human HEK293 cells, It contains, His Tag, Avitag™

Synonym
recombinant, human, biotinylated, protein VEGF121 Protein, His Tag, RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF

More details

VE1-H82E7-25

Availability: within 7 days

455,00 €

Background
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

Source
MABSol® Recombinant Biotinylated Human VEGF121, Avitag,His Tag (VE1-H82E7) is expressed from human HEK293 cells. It contains AA Ala 27 - Arg 147 (Accession # P15692-9).
Predicted N-terminus: Ala 27

Molecular Characterization
This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Angiogenesis and Functional Vessel Formation Induced by Interstitial Flow and Vascular Endothelial Growth Factor Using a Microfluidic Chip"
Liu, Li, Zhou et al
Micromachines (Basel) (2022) 13 (2)
(2) "[Application of iPDMS protein microarray in screening of tumor-associated antigen autoantibodies]"
Chen, Wang, Gu et al
Sheng Wu Gong Cheng Xue Bao (2021) 37 (11), 4075-4082
(3) "Novel engineered, membrane-tethered VEGF-A variants promote formation of filopodia, proliferation, survival, and cord or tube formation by endothelial cells via persistent VEGFR2/ERK signaling and activation of CDC42/ROCK pathways"
Shen, Rossato, Cano et al
FASEB J (2021) 35 (12), e22036
Showing 1-3 of 428 papers.

Citations

(1) "Biophysical characterization platform informs protein scaffold evolvability"
Authors: Golinski AW, et al
Journal: ACS COMB SCI 43490
Application: Binding Assay