Recombinant human spleen associated tyrosine kinase SYK, 10 µg

Background: SYK protein kinaseis widely expressed in hematopoietic cells and is involved in cellular responses to extracellular antigens and antigen-immunoglobulin complexes and thus of particular importance to the initiation of inflammatory responses. Spleen tyrosine kinase (SYK) mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). SYK is a non-receptor tyrosine kinase, which contains two SRC homology 2 (SH2)-domains and a kinase domain. Mammals also express the SYK-related molecule ZAP70, the expression of which is mostly confined to the T and NK cell lineages.

Theoretical MW (SYK) 104.4 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer:  50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.094 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 20.000 pmol/mg min

Ordering information: shipped on dry ice

Entrez Gene ID: 6850 UniProtKB: P43405


Product specific literature
Attila Mócsai, Jürgen Ruland, Victor L. J. Tybulewicz (2010) “The SYK tyrosine kinase: a crucial player in diverse biological functions” Nat Rev Immunol. 10(6): 387–402

Robert L. Geahlen (2014)  “Getting Syk: Spleen Tyrosine Kinase as a Therapeutic Target”, Trends Pharmacol Sci. 35(8): 414–422.