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Background: The SGK family belongs to the AGC class of kinases and consists of three members; SGK1, SGK2 and SGK3. While SGK1 and SGK3 are ubiquitously expressed in all tissues, SGK2 is primarily expressed only in liver, kidney, and pancreas. SGK protein kinases require activation by phosphorylation, which is accomplished through a signaling cascade by insulin and insulin-like growth factor-1 (IGF-1) involving PI 3-kinase and the 3-phosphoinositide (PIP3)-dependent kinase PDK1 and PDK2. SGK2 is a potent regulator of ion channel activity, e.g. potently stimulates ENaC activity in Xenopus laevis oocytes, transport, and transcription.
Recombinant human protein kinase SGK2 (serum/glucocorticoid regulated kinase 2), amino acids 1-367, active enzyme, N-terminally fused to GST
Theoretical MW : 68 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH 7.5
Protein concentration: 0.923 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: off-chip mobility shift assay using SGKtide as substrate and 100 µM ATP
Entrez Gene ID: 10110
UniProtKB: Q9HBY8
Ordering informations: shipped on dry ice
Pao AC.(2012) "SGK regulation of renal sodium transport."Curr Opin Nephrol Hypertens. 21(5):534-40.
Lang F, Cohen P. (2001) "Regulation and physiological roles of serum- and glucocorticoid-induced protein kinase isoforms."Sci STKE. 13;2001(108)
Kobayashi T1, Deak M, Morrice N, Cohen P. (1999) "Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase."Biochem J. 15;344 Pt 1:189-97.
Lang F1, Böhmer C, Palmada M, Seebohm G, Strutz-Seebohm N, Vallon V. (2006) "(Patho)physiological significance of the serum- and glucocorticoid-inducible kinase isoforms."Physiol Rev. 2006 Oct;86(4):1151-78.
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