Frequently Asked Questions (FAQ)

Welcome to the FAQs section of our Web site, where we provide answers to some of the questions most frequently asked by our visitors.

Nearly all of the information provided in this section can be found elsewhere on the site, and in each case, appropriate links have been provided. You may also wish to consult the Sitemap, a listing by category of all the topics covered on the site.

• ATP determination kits
  1. How do I have to calibrate the assay for exact ATP determination with a standard curve?
  2. We do not have a luminescent reader - can I use the ATP determination kits nevertheless?
  3. Which assay - time stable or sensitive - works better with cell culture samples?
  4. Regarding the samples preparation (extraction method), which type of buffer can I use?
  5. How much cell lysate do I have to add to the ATP reagent mix for ATP determination in bacteria or cell cultures?
  6. What standard protocols for ATP determination in bacteria or cell cultures do you suggest?
  7. Which components in my cell lysis buffer interfere with the luciferase reaction in your ATP determination kits?
  8. Are there other extraction methods of ATP in bacterial cultures?
  9. Are there any additional advices when working with tissue samples?
  10. Can I use the ATP determination kits for enzymatic reactions and for binding studies of ATP to recombinant proteins?
  11. Do you provide a literature list of publications for the use of the luciferase reaction for ATP determination?
  12. Can I contact somebody for special applications of the ATP determination kits not mentioned in the FAQ list?