ATP determination using the time stable bioluminescent luciferase assay. This assay is optimized for applications where ATP concentrations ranging from 10 nM up to 10 µM are determined. The luminescence signal is stable for at least 4 hours.

The luciferase bioluminescent assay includes thermostable firefly luciferase, D-Luciferin as substrate and appropriate buffer solutions optimized for sensitive ATP quantification.

Frequently Asked Questions (FAQ)

Literature References:

Kricka LJ. (1988) "Clinical and biochemical applications of luciferases and luciferins", Anal Biochem. 175 (1): 14-21

Crouch SP et al. (1993) "The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity" J Immunol Methods 160(1):81-8

Koop A et al. (1993) "Continuous bioluminescent monitoring of cytoplasmic ATP in single isolated rat hepatocytes during metabolic poisoning" Biochem J. 295 (Pt1):165-70

Petty RD et al. (1995) "Comparison of MTT and ATP-based assays for the measurement of viable cell number", J Biolumin Chemilumin. 10(1):29-34

Ronner P et al. (1999) "Luminometric assay of ATP, phosphocreatine, and creatine for estimation of free ADP and free ATP" Anal Biochem. 275(2):208-16

Sakakibara T et al. (1999) "An enzymatic cycling method using pyruvate orthophosphate dikinase and firefly luciferase for the simultaneous determination of ATP and AMP", Anal Biochem. 268(1):94-101

Price*: EUR 75,00

Availability: Product promptly available from stock

This product was added to our catalog on Dec 19, 2006


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