Recombinant, human Platelet-derived growth factor receptor alpha (PDGFRa), protein kinase domain, 10 µg

Background: The Platelet-derived growth factor (PDGF) family, characterized as a mitogen for fibroblasts and cells of mesenchymal origin in the late seventies, consists of five isoforms that are homodimers of A, B, C, and D polypeptide chains (forming PDGF-AA, -BB, -CC, and -DD, and a heterodimer PDGF-AB). The PDGF isoforms exert their cellular effects by binding to α and β‐ tyrosine kinase PDGF receptors. The PDGFR belong to the receptor-tyrosine kinase family, more precisely to the type III group, which also includes c-KIT, FLT3 and the macrophage-colony-stimulating factor receptor. PDGFRα binds to all ligands but PDGF-D, the PDGFRβ binds to PDGF-B and -D only. The two PDGF receptors are both consisting of five extracellular immunoglobulin-like domains, a single-spanning transmembrane domain and an intracellular split kinase domain, which is divided in two lobes connected by a flexible polypeptide linker, the kinase insert. Ligand induced receptor dimerization allows autophosphorylation in trans between the receptor subunits in the dimer. PDGFR-α and PDGFR-β engage several well-characterized signaling pathways e.g. Ras-MAPK, PI3K, and PLC-γ—which are known to be involved in multiple cellular and developmental responses. PDGF receptors interact also with integrins, which enhances cell proliferation, migration, and survival.

Recombinant human PDGFR-alpha amino acids Q551-L1089 (as in GenBank entry NM_006206)*, N-terminally fused to GST-FactorXa cleavage site

Theoretical MW : 87.9 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Activation: pre-incubation with 0.1 mM ATP, followed by (NH4)2SO4 precipitation
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.167mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 10,000 pmol/mg x min

Entrez Gene ID: 5156
UniProtKB: P16234

Ordering information: shipped on dry ice

Product specific literature

Demoulin JB1, Montano-Almendras CP. (2012) "Platelet-derived growth factors and their receptors in normal and malignant hematopoiesis."Am J Blood Res. 2(1):44-56.

Ostman A1, Heldin CH. (2007) “PDGF receptors as targets in tumor treatment.”Adv Cancer Res.;97:247-74.

Andrae J, Gallini R, Betsholtz C. (2008) „Role of platelet-derived growth factors in physiology and medicine.” Genes Dev. 2008 May 15;22(10):1276-312.