Recombinant Human MMP1 Protein, His tag, 50µg

Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling. Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase. MMP­1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP­1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L­Selectin, pro­TNF, IL­1β, IGF­BP3, IGF­BP5, pro MMP­2 and pro MMP­9.

Source
Recombinant Human MMP-1, His Tag (MM1-H5222) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Asn 469 (Accession # NP_002412.1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.
Predicted N-terminus: Phe 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 53.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhMMP1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, pH6.0. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Development of double strand RNA mPEI nanoparticles and application in treating invasive breast cancer"
Liu, Mu, Bai et al
RSC Adv (2019) 9 (23), 13186-13200
(2) "Elevated Plasma Matrix Metalloproteinase-8 associates with Sputum Culture Positivity in Pulmonary Tuberculosis"
Walker, Karim, Moosa et al
J Infect Dis (2022)
(3) "SLAMF8 promotes the proliferation and migration of synovial fibroblasts by regulating the ERK/MMPs signalling pathway"
Liu, Huang, Zeng et al
Autoimmunity (2022)
Showing 1-3 of 7192 papers.