Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Cynomolgus FcRN & B2M Heterodimer Protein, Strep II-tag, His-tag, 4x250 µg  

Recombinant Cynomolgus FcRN & B2M Heterodimer Protein, Strep II-tag, His-tag, 4x250 µg

Recombinant Cynomolgus FCGRT & B2M heterodimer protein (Ala 24 - Ser 297, FCGRT, Q8SPV9) and & Ile 21 - Met 119 (B2M, Q6V7J5) was produced in human 293 cells (HEK293), Strep II-tag, His-tag, SPR verified

Synonyms: recombinant, cynomolgus, protein FcRN & B2M, FCGRT & B2M

More details

FCM-C5284-1K

2 400,00 €

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified), is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)).Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization
Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 36 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation. Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (SPR verified) sequences are identical.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Immobilized Cynomolgus / Rhesus macaque FcRn Heterodimer Protein (Cat. No. FCM-C5284 ) on CM5 Chip via anti-His antibody, can bind  Herceptin with an affinity constant of 0.403 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

References

(1) Goebl NA., et al., 2008, Mol. Biol. Cell 19 (12): 5490–505.
(2) Lee TY., et al., 2008, Clin. Cancer Res. 14 (5): 1487–93.
(3) Güssow D., et al., 1987, J. Immunol. 139 (9): 3132–8.
(4) Gorevic P.D., et al., 1986, Proc. Natl. Acad. Sci. U.S.A. 83:7908-7912.

The following products could also be interesting for you: