Recombinant rat FcRn/FCGRT & B2M Heterodimer Protein (MALS & SPR verified), 1mg

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
Recombinant Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-R5287) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P13599-1(FCGRT) & P07151-1(B2M)).
Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)

Molecular Characterization
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 43-55 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Rat FcRn / FCGRT & B2M by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Bioactivity
Please refer to product data sheet.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 12 months under sterile conditions after reconstitution.

References

(1) "Polymorphisms of neonatal Fc receptor in cynomolgus and rhesus macaques"
Uno, Utoh, Iwasaki
Drug Metab Pharmacokinet (2014) 29 (5), 427-30
(2) "Humanized FcRn mouse models for evaluating pharmacokinetics of human IgG antibodies"
Proetzel, Roopenian
Methods (2014) 65 (1), 148-53
Showing 1-2 of 2 papers.
Please refer to product data sheet.