Recombinant human BAFFR / TNFRSF13C Protein, Fc Tag, 100 µg

Background
BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

Source
Recombinant human BAFFR / TNFRSF13C, Fc Tag (BAR-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).
Predicted N-terminus: Ser 7

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 33.2 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Human BAFFR / TNFRSF13C, Fc Tag (Catalog # BAR-H5257) ELISA bioactivity
Immobilized Human BAFF, His Tag (Catalog # BAF-H5248) at 5 μg/mL (100 μL/well) can bind Human BAFFR, Fc Tag (Catalog # BAR-H5257) with a linear range of 2-31 ng/mL.

References

(1) Thompson JS., et al.,2001, Science, 293 (5537): 2108-2111.
(2) Mbrose CM., 2003, Journal of Biological Regulators and Homeostatic Agents, 16 (3): 211-213.
(3) Treml LS., et al., 2006, Seminars in Immunology, 18 (5): 297-304.