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Recombinant Human Siglec-3 / CD33 Protein, Llama IgG2b Fc Tag, low endotoxin, 2 x 500 µg  

Recombinant Human Siglec-3 / CD33 Protein, Llama IgG2b Fc Tag, low endotoxin, 2 x 500 µg

Recombinant human Siglec-3, Asp 18 - His 259, expressed from human 293 cells (HEK293), Llama IgG2b Fc Tag

Synonym
recombinant, human, protein, CD33, SIGLEC3, gp67

More details

CD3-H5259-1K

1 800,00 €

Background
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

Source
Recombinant Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (CD3-H5259) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18

Molecular Characterization
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 54.6 kDa. The protein migrates as 64-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.01 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (Cat. No.CD3-H5259) at 5 μg/mL (100 μL/well) can bind Anti-CD33, Human IgG1 with a linear range of 0.078-1.25 μg/mL (QC tested).

References

(1) Garnache-Ottou F., et al., 2005, Blood 105 (3): 1256–64.
(2) Hernández-Caselles T, et al., 2006, J. Leukoc. Biol. 79 (1): 46–58.
(3) Walter RB, et al., 2007, Blood 109 (10): 4168–70.
(4) Ulyanova, T. et al., 1999, Eur. J. Immunol. 29:3440.
(5) Crocker, P.R. and A. Varki, 2001, Immunology 103:137.