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Recombinant Biotinylated Human ACE2 / ACEH Protein, Fc, Avitag™, 200µg  

Recombinant Biotinylated Human ACE2 / ACEH Protein, Fc, Avitag™, 200µg

Recombinant Biotinylated Human ACE2 / ACEH Protein,  AA Gln 18 - Ser 740, expressed from human 293 cells (HEK293), Fc Tag, Avitag™

Synonyms: Recombinant, protein, ACE-2, ACEH, ACE2

More details

AC2-H82F9-200

Availability: within 7 days

1 570,00 €

Background
Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Source
Recombinant Biotinylated Human ACE2, Fc,Avitag (AC2-H82F9) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
Predicted N-terminus: Gln 18

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 111.7 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Turner AJ., et al., 2002, Can. J. Physiol. Pharmacol. 80 (4): 346–53.
(2) Katovich MJ., et al., 2005, Exp. Physiol. 90 (3): 299–305.
(3) Donoghue M., et al., 2000, Circ. Res. 87:E1-E9.
(4) Tipnis S.R., et al., 2000, J. Biol. Chem. 275:33238-33243.
(5) Vickers C., et al., 2002, J. Biol. Chem. 277:14838-14843.

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