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Recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag, His Tag, 2x 500µg  

Recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag, His Tag, 2x 500µg

Recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, AA Met 1 - Ala 419, expressed from E.coli cells, His Tag

Synonyms: Recombinant, Nucleocapsid protein, NP, Protein N, COVID-19

More details

NUN-C51H9-1K

Availability: within 7 days

2 600,00 €

Background
Nucleocapsid protein is a most abundant protein of coronavirus. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we identified as the viral nucleocapsid (N) protein. Novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein. The array of diverse functional activities accommodated in the hantaviral N protein goes far beyond to be a static structural protein and makes it an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Source
Recombinant 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag (NUN-C51H9) is expressed from E.coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 49.4 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Reuter M, et al. Virus Genes. 2018. 54(1):5-16.
(2) Grunewald ME, et al. Virology. 2018. 517:62-68.
(3) Jeeva S, et al. PLoS One. 2017. 12(9):e0184935.

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