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Recombinant 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag, HEK293 expressed, 1mg  

Recombinant 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag, HEK293 expressed, 1mg

Recombinant 2019-nCoV (COVID-19) Nucleocapsid protein, AA Met 1 - Ala 419, expressed from human 293 cells (HEK293, His Tag

Synonyms: Recombinant, Nucleocapsid protein, NP, Protein N, COVID-19

More details

NUN-C5227-1K

Availability: within 10-14 days

2 600,00 €

Background
Nucleocapsid protein is a most abundant protein of coronavirus. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we identified as the viral nucleocapsid (N) protein. Novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein. The array of diverse functional activities accommodated in the hantaviral N protein goes far beyond to be a static structural protein and makes it an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Source
Recombinant  2019-nCoV (COVID-19) Nucleocapsid protein, His Tag (NUN-C5227) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # YP_009724397.2). Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Reuter M, et al. Virus Genes. 2018. 54(1):5-16.
(2) Grunewald ME, et al. Virology. 2018. 517:62-68.
(3) Jeeva S, et al. PLoS One. 2017. 12(9):e0184935.

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