Recombinant SARS S1 protein, His Tag (MALS verified), 100µg

Background
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS S1 protein, His Tag (S1N-S52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1).
Predicted N-terminus: Ser 14

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Investigation of IgG Titers in Hemodialysis Patients and Controls Following Administration of the COVID-19 Vaccine"
Jozpanahi, Jafari, Moharrer et al
Int Tinnitus J (2024) 27 (2), 183-190
(2) "Functional dissection of the spike glycoprotein S1 subunit and identification of cellular cofactors for regulation of swine acute diarrhea syndrome coronavirus entry"
Yang, Wang, Li et al
J Virol (2024)
(3) "Characteristics of neutralizing antibody titers of the SARS-CoV-2 vaccine in maintenance hemodialysis patients"
Amari, Morimoto, Teranishi et al
Clin Nephrol (2024)
Showing 1-3 of 2946 papers.