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Recombinant SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified), 500µgx2  

Recombinant SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified), 500µgx2

Recombinant SARS-CoV-2 (COVID-19) S1 protein, AA Val 16 - Arg 685, expressed from human 293 cells (HEK293), MALS verified, His Tag

Synonyms: Recombinant, protein, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, COVID-19

More details

S1N-C52H4-1K

Availability: within 7 days

3 000,00 €

Background
Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant 2019-nCoV S1 protein, His Tag (S1N-C52H4) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). Predicted N-terminus: Val 16

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
(2) Benvenuto D, et al. J Med Virol. 2020.
(3) Chang CY, et al. AMB Express. 2020. 10(1):20.

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