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Recombinant SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag, 5x 200µg  

Recombinant SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag, 5x 200µg

Recombinant SARS-CoV-2 (COVID-19) NSP1 Protein, AA Met 1 - Gly 180, expressed from E. coli cells, His Tag

Synonyms: Recombinant, protein, NSP1, nsp1, Leader protein, Host translation inhibitor nsp1, COVID-19

More details

NS1-C51H7-1K

Availability: within 10-14 days

2 600,00 €

Background
The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.

Source
Recombinant SARS-CoV-2 NSP1, His Tag (NS1-C51H7) is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Dong S, et al. J Med Virol. 2020.(2) Gottipati K, et al. Virology. 2020. 544:31-41.
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