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Recombinant SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag, 100µg  

Recombinant SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag, 100µg

Recombinant SARS-CoV-2 (COVID-19) NSP8 Protein, AA Ala 1 - Gln 198, expressed from E. coli cells, His Tag

Synonyms: Recombinant, protein, NSP8, nsp8, Non-structural protein 8, COVID-19

More details

NS8-C5149-100

Availability: within 7 days

480,00 €

Background
NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of SARS-CoV-2, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity.

Source
Recombinant SARS-CoV-2 NSP8, His Tag (NS8-C5149) is expressed from E.coli cells. It contains AA Ala 1 - Gln 198 (Accession # YP_009725304.1). Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.9 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Delivered as bulk protein in a 0.2 μm filtered solution of 20 mM Tris, 250 mM NaCl, pH8.5 with glycerol as protectant.

Bioactivity
Please refer to product data sheet.

Storage
The product MUST be stored at -70°C or lower upon receipt;
-70°C for 3 months under sterile conditions.

References

(1) te Velthuis AJ, et al. Nucleic Acids Res. 2012. 40(4):1737-47.(2) Edison Ong, et al. doi.org. 2020. 000141.
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