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Recombinant SARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, His Tag, 5x 200ug  

Recombinant SARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, His Tag, 5x 200ug

Recombinant SARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, AA Ser 1 - Gln 83 (NSP7) & Ala 1 - Gln 198 (NSP8), expressed from E. coli cells, His Tag

Synonyms: Recombinant, protein, NSP7 & NSP8, nsp7 & nsp8, Non-structural protein 8, Non-structural protein 7, COVID-19

More details

NS8-C5125-1K

Availability: within 10-14 days

2 600,00 €

Background
During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.

Source
Recombinant SARS-CoV-2 NSP7&NSP8, His Tag (NS8-C5125) is expressed from E.coli cells. It contains AA Ser 1 - Gln 83 (NSP7) & Ala 1 - Gln 198 (NSP8) (Accession # YP_009725303.1 (NSP7) & YP_009725304.1 (NSP8)).Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag. The protein has a calculated MW of 32.1 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Delivered as bulk protein in a 0.2 μm filtered solution of PBS, pH7.4 with glycerol as protectant.

Bioactivity
Please refer to product data sheet.

Storage
The product MUST be stored at -70°C or lower upon receipt;
-70°C for 3 months under sterile conditions.

References

(1) Krichel B, et al. Biochem J. 2020. 477(5):1009-1019.(2) Kirchdoerfer RN, et al. Nat Commun. 2019. 10(1):2342.
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