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Recombinant SARS-CoV-2 (COVID-19) S2 protein, Mouse IgG1 Fc Tag, 4x 250µg  

Recombinant SARS-CoV-2 (COVID-19) S2 protein, Mouse IgG1 Fc Tag, 4x 250µg

Recombinant SARS-CoV-2 (COVID-19) S2 protein, AA Ser 686 - Pro 1213, expressed from human 293 cells (HEK293), Mouse IgG1 Fc Tag

Synonyms: Recombinant, protein, S2 protein, Spike glycoprotein Subunit2, S glycoprotein Subunit2, Spike protein S2, COVID-19

More details

S2N-C52Ac-1K

Availability: within 10-14 days

2 600,00 €

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 S2 protein, Mouse IgG1 Fc Tag (S2N-C52Ac) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). Predicted N-terminus: Ser 686

Molecular Characterization
This protein carries a mouse IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 84.1 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
(2) Benvenuto D, et al. J Med Virol. 2020.
(3) Chang CY, et al. AMB Express. 2020. 10(1):20.

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