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Recombinant SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His Tag, 100µg  

Recombinant SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His Tag, 100µg

Recombinant SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), AA Val 16 - Arg 685, HV69-70 deletion, expressed from human 293 cells (HEK293), His Tag

Synonyms: Recombinant, protein, Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, COVID-19

More details

S1N-C52Hd-100

Availability: within 7 days

456,00 €

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 S1 protein (HV69-70del), His Tag (S1N-C52Hd) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1(HV69-70del)). HV69-70 deletion was identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom.
Predicted N-terminus: Val 16

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 76.7 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

  1. Zanin, Saraceno, Renisi et al. (2021)"Delayed onset of fatal encephalitis in a COVID-19 positive patient". Int J Neurosci.
  2. Xu, Liu, Xiao et al. (2021) "Computational and Experimental Studies Reveal That Thymoquinone Blocks the Entry of Coronaviruses Into In Vitro Cells". Infect Dis Ther.
  3. Barrett, Neal, Edmonds et al. (2021) "Effect of mutations in the SARS-CoV-2 spike protein on protein stability, cleavage, and cell-cell fusion function". bioRxiv
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