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Recombinant SARS-CoV-2 Nucleocapsid protein (P80R), His Tag, 100µg  

Recombinant SARS-CoV-2 Nucleocapsid protein (P80R), His Tag, 100µg

Recombinant SARS-CoV-2 Nucleocapsid protein (P80R), AA Met 1 - Ala 419, expressed from human 293 cells (HEK293), His Tag

Synonyms: Recombinant, Nucleocapsid protein, NP, Protein N

More details

NUN-C52Hc-100

Availability: within 10-14 days

480,00 €

Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Source
Recombinant SARS-CoV-2 Nucleocapsid protein (P80R), His Tag (NUN-C52Hc) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P80R)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation P80R was identified on the nucleocapsid protein of SARS-CoV-2 variants with high frequency in P.1 variants circulating in Brazil (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 47.4 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

Please refer to product data sheet.