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GMP Human IL-1 beta Protein, 50µg  

GMP Human IL-1 beta Protein, 50µg

GMP Human IL-1 beta Protein, AA Ala 117 - Ser 269, expressed from E. coli cells, (Bioactivity and Stability tested)

Designed under ISO 9001:2015 and ISO 13485:2016, Manufactured and QC tested under a GMP compliance factory, FDA DMF filed

More details

GMP-ILBH16-50

Availability: within 7 days

767,00 €

Background
Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The mature human IL1β shares 96 % amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1β.

Features
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Batch-to-batch consistency
- Stringent quality control tests
- No animal derived peptone and lactose used in production process

Source
GMP Human IL-1 beta Protein(GMP-ILBH16) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1 ). Predicted N-terminus: Met

Molecular Characterization
This protein carries no "tag". The protein has a calculated MW of 17.5 kDa. The protein migrates as 18 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 10 EU per mg by the LAL method.

Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA
<0.02 ng/μg of protein tested by DNA Fluorescent Staining method.

Sterility
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.

Mycoplasma
Negative.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
Shipping at ambient temperature. Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 5 years in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) Shen, Li, Wang et al., "ZIP4 upregulation aggravates nucleus pulposus cell degradation by promoting inflammation and oxidative stress by mediating the HDAC4-FoxO3a axis", Aging (Albany NY) (2024) 15
(2) Xie, Wu, He et al., "A slow-releasing donor of hydrogen sulfide inhibits neuronal cell death via anti-PANoptosis in rats with spinal cord ischemia‒reperfusion injury", Cell Commun Signal (2024) 22 (1), 33
(3) Chen, Cui, Cui et. al, "Vagus nerve stimulation attenuates septic shock-induced cardiac injury in rats" , Physiol Res (2023) 72 (6), 731-739