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GMP Human SCF Protein, 50µg  

GMP Human SCF Protein, 50µg

GMP Human SCF Protein, AA Glu 26 - Ala 189, expressed from human 293 cells (HEK293), (Bioactivity and Stability tested)

Designed under ISO 9001:2015 and ISO 13485:2016, Manufactured and QC tested under a GMP compliance factory, FDA DMF filed

More details

GMP-SCFH25-50

Availability: within 7 days

780,00 €

Background
Stem Cell Factor is also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, and is a cytokine that binds to the c-Kit receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same R transcript.Soluble and transmembrane SCF is produced by fibroblasts and endothelial cells. Soluble SCF has a molecular weight of 18,5 KDa and forms a dimer. SCF plays an important role in the hematopoiesis during embryonic development. Sites where hematopoiesis takes place, such as the fetal liver and bone marrow, all express SCF. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo (outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation.

Features
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Batch-to-batch consistency
- Stringent quality control tests
- No animal derived peptone and lactose used in production process

Source
GMP Human SCF Protein(GMP-SCFH25) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 189 (Accession # P21583-1 ). Predicted N-terminus: Glu 26

 

Molecular Characterization
This protein carries no "tag". The protein has a calculated MW of 18.5 kDa. The protein migrates as 30 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 10 EU per mg by the LAL method.

Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA
<0.02 ng/μg of protein tested by DNA Fluorescent Staining method.

Sterility
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.

Mycoplasma
Negative.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
Shipping at ambient temperature. Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 5 years in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) Pascale, D'Arco, Lebègue et al., "Jahn-Teller distortion, octahedra rotations and orbital ordering in perovskites: KScF 3
as a model system", J Comput Chem (2024)
(2) Chen, Wang, Dai et al., "FBXO31 is upregulated by METTL3 to promote pancreatic cancer progression via regulating SIRT2 ubiquitination and degradation", Cell Death Dis (2024) 15 (1), 37
(3) Wang, Zhou, Xu et al., "Effects of stem cell factor in follicular fluid and granulosa cells on oocyte maturity and clinical pregnancy", Medicine (Baltimore) (2023) 102 (52), e36749