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GMP Biotinylated Human DLL4 Protein, His,Avitag™, 200µg  

GMP Biotinylated Human DLL4 Protein, His,Avitag™, 200µg

GMP Biotinylated Human DLL4 Protein, AA Ser 27 - Pro 524, expressed from human 293 cells (HEK293), His,Avitag™, (Bioactivity and Stability tested)

Designed under ISO 9001:2015 and ISO 13485:2016, Manufactured and QC tested under a GMP compliance factory, FDA DMF filed

More details

GMP-DL4H23-200

Availability: within 7 days

1 235,00 €

Background
Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.

Features
- Designed under ISO 9001:2015 and ISO 13485:2016
- Manufactured and QC tested under a GMP compliance factory
- FDA DMF filed
- Animal-Free materials
- Batch-to-batch consistency
- Stringent quality control tests
- No animal derived peptone and lactose used in production process

Source
GMP Biotinylated Human DLL4 Protein, His,Avitag(GMP-DL4H23) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # NP_061947.1 ). Predicted N-terminus: Ser 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 57.9 kDa. The protein migrates as 61 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotinylation
As determined by Quantative ELISA binding assay against streptavidin.

Endotoxin
Less than 10 EU per mg by the LAL method.

Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA
<0.02 ng/μg of protein tested by DNA Fluorescent Staining method.

Sterility
The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1.

Mycoplasma
Negative.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with protectants.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
Shipping at ambient temperature. Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 5 years in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Assessing angiogenesis factors as prognostic biomarkers in breast cancer patients and their association with clinicopathological factors"
Abbasi-Dokht, Malek, Nafissi et al
Biomarkers (2024)
(2) "Retracted: Analysis of Molecular Mechanism of YiqiChutan Formula Regulating DLL4-Notch Signaling to Inhibit Angiogenesis in Lung Cancer"
International
Biomed Res Int (2024) 2024, 9894642
(3) "The phosphodiesterase 2A controls lymphatic junctional maturation via cGMP-dependent notch signaling"
Carlantoni, Liekfeld, Hemkemeyer et al
Dev Cell (2024) 59 (3), 308-325.e11
Showing 1-3 of 1262 papers.