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Recombinant human BAFF/CD257 Protein, 50µg  

Recombinant human BAFF/CD257 Protein, 50µg

Recombinant Human BAFF /CD257 Protein (rhBAFF) Ala134-Leu285 was produced in human 293 cells (HEK293)

Synonym
TNFSF13B , BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK , TNFSF20, ZTNF4

More details

BAF-H4218-050

Availability: within 7 days

312,00 €

Background
B-cell activating factor (BAFF), also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors[1], although BAFF has been known to be active as either a hetero- or homotrimer.[2] BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

Source
Recombinant Human BAFF /CD257 Protein (rhBAFF) Ala134-Leu285 (Accession # AAH20674.1) was produced in human 293 cells (HEK293).

Molecular Characterization
rhBAFF, fused with Gly-Pro at N-terminus and has a calculated MW of 17.2 kDa. DTT-reduced protein migrates as 19 kDa polypeptide in SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg of the rhBAFF by the LAL method.

Purity
>96% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells.The ED50 for this effect is typically 0.3-1.8 ng/ml in the presence of goat antimouse IgM μ chain.

References

(1) Oren,D.A., et al., 2002, Nat. Struct. Biol. 9 (4): 288–292.
(2) Daridon C, er al.,2008, Autoimmun Rev 7 (4): 267–71