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Recombinant human Akt1 / PKB alpha protein kinase, unactive, 20 µg  

Recombinant human Akt1 / PKB alpha protein kinase, unactive, 20 µg

Recombinant human protein kinase Akt1 / PKB alpha, unphosphorylated and unactive enzyme

Alternate names: protein kinase B alpha, PKB, Akt 1, PRKBA, RAC-alpha

More details

PK-PKBA-I020

Availability: within 5-7 days

255,00 €

Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a pleckstrin homology (PH) domain. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration.

Human protein kinase Akt1/PKBalpha, recombinantly expressed in Sf9 cells. Unactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate, can be activated by upstream kinases. 

Expression system: Sf9 cells
Purification: One-step affinity purification using Ni-NTA agarose
Purity: > 90% by SDS PAGE
Theoretical MW: 59.1 kDa
Storage buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 25 mM beta-glycerophosphate, 50 % glycerol, pH 8.5.
Storage temperature: - 80°C (avoid repeated freeze-thaw cycles !)
Protein concentration: 1.9 mg/ml (Bradford with BSA as standard protein)
Specific activity: 280 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide (PRTSSFAE) per min at 25 °C. Kinase activity was measured using a microfluidic mobility shift assay.)

Entrez Gene ID: 207 
UniProtKB: P31749

Ordering information: shipped on dry ice

BIAFFIN – SERVICES:

Use the PKB / Akt - SPR Binding Assay of BIAFFIN for comprehensive kinetic characterization of your small molecule kinase inhibitors. 

AKT_MK2206.png
Figure: Real-time kinetic analysis of kinase inhibitor MK-2206 binding to PKBα / Akt1 using surface plasmon resonance.

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Product specific literature references:

  • Downward J (1998) "Mechanisms and consequences of activation of protein kinase B/Akt" Curr Opin Cell Biol. 10(2):262-7

  • Brazil DP, Hemmings BA (2001) "Ten years of protein kinase B signalling: a hard Akt to follow" Trends Biochem Sci. 26(11):657-64

  • Nicholson KM, Anderson NG (2002) "The protein kinase B/Akt signalling pathway in human malignancy" Cell Signal. 14(5):381-95

Product cititations:

  • María Llanos Valeroa, Rosario Sabariegosa,d, Francisco J. Cimasb, Celia Peralese, Esteban Domingoe, Ricardo Sánchez-Prieto and Antonio Masa (2016) "Hepatitis C Virus RNA-Dependent RNA Polymerase Interacts with the Akt/PKB Kinase and Induces Its Subcellular Relocalization" Antimicrob. Agents Chemother. vol. 60 no. 6 3540-3550