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Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a pleckstrin homology (PH) domain. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration.
Human protein kinase Akt1/PKBalpha, recombinantly expressed in Sf9 cells. Unactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate. The inactive Akt1/PKBalpha is not phosphorylated at Thr308 and Ser473 and can be used to test phosphorylation of these two sites.
Expression system: Sf9 cells
Purification: One-step affinity purification using Ni-NTA agarose
Purity: > 90% by SDS PAGE
Theoretical MW: 59.1 kDa
Storage buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 25 mM beta-glycerophosphate, 50 % glycerol, pH 8.5.
Storage temperature: - 80°C (avoid repeated freeze-thaw cycles !)
Protein concentration: 1.9 mg/ml (Bradford with BSA as standard protein)
Specific activity: 235 U*/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide (RPRAATF) per min at 30 °C)
Entrez Gene ID: 207
Ordering information: shipped on dry ice
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Downward J (1998) "Mechanisms and consequences of activation of protein kinase B/Akt" Curr Opin Cell Biol. 10(2):262-7
Brazil DP, Hemmings BA (2001) "Ten years of protein kinase B signalling: a hard Akt to follow" Trends Biochem Sci. 26(11):657-64
Nicholson KM, Anderson NG (2002) "The protein kinase B/Akt signalling pathway in human malignancy" Cell Signal. 14(5):381-95
PKB/Akt substrate, 25 mg - 250,00 €
Triciribine, 1mg - 145,00 €
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