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 bioluminescence based  ATP determination kit (sensitive, 10 ml)  

bioluminescence based ATP determination kit (sensitive, 10 ml)

ATP determination using the sensitive bioluminescent luciferase assay (10 ml assay kit)

bioluminescent luciferase assay (sensitive ATP determination kit) optimized for ATP quantification in biological samples (10 ml assay kit for 200 – 1000 assays)

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145,00 €

Biaffins ATP determination kit is a sensitive bioluminescent assay using the luciferase-luciferin system. This assay is optimized for a very sensitive detection of very small amounts of ATP in various samples. Even 0.1 pmol of ATP can accurately be determined.

The bioluminescent ATP determination assay includes thermostable firefly luciferase, D-Luciferin as substrate and appropriate buffer solutions optimized for sensitive ATP quantification.

Quantity: 10 ml assay kit for 200 - 1000 assays

Frequently Asked Questions (FAQ)

Literature References:

Kricka LJ. (1988) "Clinical and biochemical applications of luciferases and luciferins", Anal Biochem. 175 (1): 14-21

Crouch SP et al. (1993) "The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity" J Immunol Methods 160(1):81-8

Koop A et al. (1993) "Continuous bioluminescent monitoring of cytoplasmic ATP in single isolated rat hepatocytes during metabolic poisoning" Biochem J. 295 (Pt1):165-70

Petty RD et al. (1995) "Comparison of MTT and ATP-based assays for the measurement of viable cell number", J Biolumin Chemilumin. 10(1):29-34

Ronner P et al. (1999) "Luminometric assay of ATP, phosphocreatine, and creatine for estimation of free ADP and free ATP" Anal Biochem. 275(2):208-16

Sakakibara T et al. (1999) "An enzymatic cycling method using pyruvate orthophosphate dikinase and firefly luciferase for the simultaneous determination of ATP and AMP (RNA)", Anal Biochem. 268(1):94-101

Product Citations:

Krijnen PA, Cillessen SA, Manoe R, Muller A, Visser CA, Meijer CJ, Musters RJ, Hack CE, Aarden LA, Niessen HW.(2005) "Clusterin: a protective mediator for ischemic cardiomyocytes?" Am J Physiol Heart Circ Physiol.289(5):H2193-202.

Thies E, Mandelkow EM. (2007) " Missorting of tau in neurons causes degeneration of synapses that can be rescued by the kinase MARK2/Par-1." J Neurosci. 27(11):2896-907.

Krijnen PA, Sipkens JA, Molling JW, Rauwerda JA, Stehouwer CD, Muller A, Paulus WJ, van Nieuw Amerongen GP, Hack CE, Verhoeven AJ, van Hinsbergh VW, Niessen HW.(2010) "Inhibition of Rho-ROCK signaling induces apoptotic and non-apoptotic PS exposure in cardiomyocytes via inhibition of flippase." J Mol Cell Cardiol. 49(5):781-90.

Ghanbari-Niaki A, Soltani R, Shemshaki A, Kraemer RR.(2010) "Effects of acute ethionine injection on plasma ghrelin and obestatin levels in trained male rats." Metabolism. 59(7):982-7.

Lai B, Yu S, Bernhardt PV, Rabaey K, Virdis B, Krömer JO. (2015) "Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system." Biotechnol Biofuels. 18;9:39

Surova L, Sherstneva O, Vodeneev V, Katicheva L, Semina M, Sukhov V. (2016) "Variation potential-induced photosynthetic and respiratory changes increase ATP content in pea leaves." J Plant Physiol.202:57-64.

Mewes M, Nedele J, Schelleckes K, Bondareva O, Lenders M, Kusche-Vihrog K, Schnittler HJ, Brand SM, Schmitz B, Brand E. (2017) "Salt-induced Na+/K+-ATPase-α/β expression involves soluble adenylyl cyclase in endothelial cells." Pflugers Arch. 2017 May 26. doi: 10.1007/s00424-017-1999-6.