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bioluminescence based ATP determination kit (timestable, 10 ml)  

bioluminescence based ATP determination kit (timestable, 10 ml)

ATP determination using the time stable bioluminescent luciferase assay

bioluminescent luciferase assay (time stable ATP determination kit) optimized for ATP quantification in biological samples (10 ml assay kit for 200 – 1000 assays)

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125,00 €

Biaffins time stable ATP determination kit is bioluminescent assay based on the luciferase-luciferin system. This assay is optimized for applications where ATP concentrations ranging from 10 nM up to 10 µM are determined. The luminescence signal is stable for at least 4 hours.

The bioluminescent ATP determination kit includes thermostable firefly luciferase, D-Luciferin as substrate and appropriate buffer solutions optimized for sensitive ATP quantification.

Quantity: 10 ml for 200 - 1000 assays

Frequently Asked Questions (FAQ)

Literature References:

Kricka LJ. (1988) "Clinical and biochemical applications of luciferases and luciferins", Anal Biochem. 175 (1): 14-21

Crouch SP et al. (1993) "The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity" J Immunol Methods 160(1):81-8

Koop A et al. (1993) "Continuous bioluminescent monitoring of cytoplasmic ATP in single isolated rat hepatocytes during metabolic poisoning" Biochem J. 295 (Pt1):165-70

Petty RD et al. (1995) "Comparison of MTT and ATP-based assays for the measurement of viable cell number", J Biolumin Chemilumin. 10(1):29-34

Ronner P et al. (1999) "Luminometric assay of ATP, phosphocreatine, and creatine for estimation of free ADP and free ATP" Anal Biochem. 275(2):208-16

Sakakibara T et al. (1999) "An enzymatic cycling method using pyruvate orthophosphate dikinase and firefly luciferase for the simultaneous determination of ATP and AMP (RNA)", Anal Biochem. 268(1):94-101

Product Citations:

Hutchinson DS, Bengtsson T. (2006) "AMP-activated protein kinase activation by adrenoceptors in L6 skeletal muscle cells: mediation by alpha1-adrenoceptors causing glucose uptake." Diabetes.55(3):682-90.

Anghel SI, Bedu E, Vivier CD, Descombes P, Desvergne B, Wahli W.(2007) "Adipose tissue integrity as a prerequisite for systemic energy balance: a critical role for peroxisome proliferator-activated receptor gamma." J Biol Chem. 282(41):29946-57.  

Paul K, Erhardt M, Hirano T, Blair DF, Hughes KT. (2008) "Energy source of flagellar type III secretion." Nature ;451(7177):489-92. 

Jorgensen AL, Juul-Madsen HR, Stagsted J. (2010) "Colostrum and bioactive, colostral peptides differentially modulate the innate immune response of intestinal epithelial cells." J Pept Sci. 16(1):21-30.

Liebmann J, Born M, Kolb-Bachofen V. (2010) "Blue-light irradiation regulates proliferation and differentiation in human skin cells."J Invest Dermatol. 130(1):259-69.

Mewes M, Nedele J, Schelleckes K, Bondareva O, Lenders M, Kusche-Vihrog K, Schnittler HJ, Brand SM, Schmitz B, Brand E. (2017) "Salt-induced Na+/K+-ATPase-α/β expression involves soluble adenylyl cyclase in endothelial cells." Pflugers Arch. 2017 May 26. doi: 10.1007/s00424-017-1999-6.