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Recombinant human HGFR / c-MET Protein, With C-Fc Tag, 1mg  

Recombinant human HGFR / c-MET Protein, With C-Fc Tag, 1mg

Recombinant Human HGFR / c-MET Protein, with C-Fc Tag (rh c-MET /HGFR) Glu 25 - Thr 932 produced in human 293 cells (HEK293)

Synonym
recombinant human protein, MET, AUTS9, HGFR, RCCP2, c-Met

More details

MET-H5256-1K

Availability: within 7 days

1 440,00 €

Background
Hepatocyte growth factor receptor (HGFR), also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis [1-4].

Source
Recombinant Human HGFR /c-MET Protein, with C-Fc Tag (rh c-MET /HGFR) Glu 25 - Thr 932 (Accession # AAI30421.1) was produced in human 293 cells (HEK293).

Molecular Characterization
rhHGFR is fused with Fc fragment of human IgG1 at the C-terminus. The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved ˞ and ˟ chain. Each ˞ and ˟ chain has a calculated MW of 32.5 kDa (˞ chain) and 96.7 kDa (˟ chain Fc chimera). The predicted N-terminal is Glu25 (˞ chain) & Ser308 (˟ chain Fc chimera). Protein migrates as 45 kDa (˞ chain) and 120-125 kDa (˟chain Fc chimera) in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh c-MET /HGFR by the LAL method.

Purity
>95% as determined by SDS-PAGE. The purity of rh c-MET /HGFR was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity
N/A

References

(1) Bottaro DP, et al., 1991, Science 251 (4995): 802–4.
(2) Galland F, et al., 1992, Cytogenet. Cell Genet. 60 (2): 114–6.
(3) Cooper CS, 1992, Oncogene 7 (1): 3–7.
(4) Gentile A, et al., 2008, Cancer Metastasis Rev. 27 (1): 85–94.