kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human PCSK9 Protein, His Tag, 1mg  

Recombinant human PCSK9 Protein, His Tag, 1mg

Recombinant human PCSK9(rhPCSK9), produced in human HEK293 cells, His Tag

recombinant human protein, PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9

More details


Availability: within 7 days

2 178,00 €

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons.(1) Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).(2,3).

Recombinant human PCSK9 protein (rhPCSK9) Gln31-Gln692 (Accession # AAI66619) was produced in human HEK293 cells

Molecular Characterization
rhPCSK9 contains C-terminal polyhistidine tag and has a calculated MW of 75.1 kDa. As a result of glycosylation and proteolytic digestion, DTT-reduced protein migrates as 20 kDa and 62 kDa polypeptide in SDS-PAGE.

Less than 1.0 EU per 1 μg of the rhPCSK9 by the LAL method.

>97% as determined by SDS-PAGE. All lots are greater than 97% pure.

Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally trehalose and mannitol are added as protectants before lyophilization.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with a linear range of 0.031-0.125 μg/mL (QC tested).


(1) Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928–33.
(2) Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
(3) Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454–9.