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Recombinant human PCSK9 Protein, His Tag, 1mg  

Recombinant human PCSK9 Protein, His Tag, 1mg

Recombinant human PCSK9(rhPCSK9), produced in human HEK293 cells, His Tag

Synonym
recombinant human protein, PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9

More details

PC9-H5223-1K

Availability: within 7 days

1 980,00 €

Background
Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons.(1) Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).(2,3).

Source
Recombinant human PCSK9 protein (rhPCSK9) Gln31-Gln692 (Accession # AAI66619) was produced in human HEK293 cells

Molecular Characterization
rhPCSK9 contains C-terminal polyhistidine tag and has a calculated MW of 75.1 kDa. As a result of glycosylation and proteolytic digestion, DTT-reduced protein migrates as 20 kDa and 62 kDa polypeptide in SDS-PAGE.

Endotoxin
Less than 1.0 EU per 1 μg of the rhPCSK9 by the LAL method.

Purity
>97% as determined by SDS-PAGE. All lots are greater than 97% pure.

Formulation
Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally trehalose and mannitol are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with a linear range of 0.031-0.125 μg/mL (QC tested).

References

(1) Seidah NG, et al., Proc. Natl. Acad. Sci. U.S.A. 100 (3): 928–33.
(2) Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
(3) Dubuc G. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (8): 1454–9.