Recombinant Human BMPR-1A / ALK-3 Protein, His Tag (MALS verified), 200µg

Background
The bone morphogenetic protein receptor, type IA also known as BMPR1A or ALK3 is a protein which in humans is encoded by the BMPR1A gene. BMPR1A has also been designated as CD292 (cluster of differentiation 290). The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A (this protein) and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2.[1] Alk3-mediated BMP signaling in AV endocardial/mesenchymal cells plays a central role during cushion morphogenesis.[2]

Source
Recombinant Human BMPR-1A, His Tag (AL3-H5228) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Arg 152 (Accession # P36894-1).
Predicted N-terminus: Gln 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.1 kDa. The protein migrates as 25-29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product dat asheet.

Clinical and Translational Updates

(1) "BMPR1A is necessary for chondrogenesis and osteogenesis, whereas BMPR1B prevents hypertrophic differentiation"
Mang, Kleinschmidt-Doerr, Ploeger et al
J Cell Sci (2020) 133 (16)
(2) "Transforming growth factor-beta family members are regulated during induced luteolysis in cattle"
Haas, Rovani, Ilha et al
Anim Reprod (2019) 16 (4), 829-837
(3) "A study of Drynaria fortunei in modulation of BMP–2 signalling by bone tissue engineering"
Dong, Ma, Li et al
Turk J Med Sci (2020) 50 (5), 1444-1453
Showing 1-3 of 40 papers.