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Recombinant human IGFBP3 Protein, His tag, 100µg  

Recombinant human IGFBP3 Protein, His tag, 100µg

Recombinant human IGFBP-3 Protein (rhIGFBP3), Gly28-Lys291, expressed HEK 293 Cells, His Tag

Synonym
Recombinant human protein, IGFBP3, IGFBP-3, BP-53, IBP3, IBP-3

More details

IG3-H5229-100

Availability: within 7 days

420,00 €

Background
Insulin-like growth factor-binding protein 3, also known as IGFBP3, is a protein that, in humans, is encoded by the IGFBP3 gene.[1][2] IGFBP3 forms a ternary complex of about 140 ~150 kDa with IGF1 or IGF2 and a glycoprotein insulin-like growth factor acid-labile subunit (ALS), thus alter the interaction of IGFs with their cell surface receptors. IGFBP3 exerts either proapoptotic or growth stimulatory effects depending upon the cellular context. Studies have shown that IGFBP3 can leads to the induction of apoptosis dependent or independent of the IGF-IGF receptor axis, accordingly acts as a negative regulator of tumorigenesis and progressing in certain carcinomas. The highest expression level is found in the nonparanchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty.

Source
Recombinant Human IGFBP-3, His Tag (IG3-H5229) is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # AAH18962).
Predicted N-terminus: Gly 28

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 31.0 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per 1 μg of the rhIGFBP3 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Trehalose and mannitol are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity-ELISA
Immobilized Human IGF-I, Fc Tag (Cat. No. IG1-H4269) at 5 μg/mL (100 μL/well) can bind Human IGFBP-3, His Tag (Cat. No. IG3-H5229) with a linear range of 0.031-0.5 μg/mL (QC tested).

Immobilized Human IGFBP-3, His Tag (Cat. No. IG3-H5229) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-II, Avitag,Fc Tag (Cat. No. IG2-H82F9) with a linear range of 0.008-0.1 μg/mL (Routinely tested).

References

(1) Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3
(2) Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619
(3) Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1