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Recombinant human DKK-1 /DKK1 Protein, His tag, 2x 500µg  

Recombinant human DKK-1 /DKK1 Protein, His tag, 2x 500µg

Recombinant Human DKK-1 /DKK1 Protein (Thr32-His266), expressed in human 293 cells (HEK293), His tag

Synonym: recombinant, human, protein DKK-1, DKK1

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Availability: within 7 days

1 800,00 €

Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts(1). DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma(2). More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression(3).

Recombinant Human Dkk-1, His Tag (DK1-H5221) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). Predicted N-terminus: Thr 32

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg of the rhDKK1 by the LAL method.

>95% as determined by SDS-PAGE of reduced rhDKK1.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.01-0.156 μg/mL (QC tested).


(1) Li Y, Lu W, et al.( 2010), PLoS One.Jun 8;5(6):e11014
(2) Yamaguchi Y, Morita A, et al.(2009).J Investig Dermatol Symp Proc. Aug;14(1):73-5.
(3) Lieven O, Rüther al.(2011). Dev Biol. Apr 22.