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Recombinant human DKK-1 /DKK1 Protein, His tag, 2x 500µg  

Recombinant human DKK-1 /DKK1 Protein, His tag, 2x 500µg

Recombinant Human DKK-1 /DKK1 Protein (Thr32-His266), expressed in human 293 cells (HEK293), His tag (MALS verified)

Synonym: recombinant, human, protein DKK-1, DKK1, SK

More details

DK1-H5221-1K

Availability: within 7 days

1 800,00 €

Background
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts(1). DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma(2). More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression(3).

Source
Recombinant Human Dkk-1, His Tag (DK1-H5221) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). Predicted N-terminus: Thr 32

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhDKK1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Effects of baicalin on the proliferation and expression of OPG and RANKL in human cementoblast-lineage cells"
Kunimatsu, Kimura, Sakata et al
J Dent Sci (2022) 17 (1), 162-169
(2) "Biological and molecular profile of fracture non-union tissue: A systematic review and an update on current insights"
Panteli, Vun, Pountos et al
J Cell Mol Med (2022)
(3) "Bone mineral density, bone metabolism-related factors, and microRNA-218 are correlated with disease activities in Chinese ankylosing spondylitis patients"
Liu, Zhao, Yang et al
J Clin Lab Anal (2022)
Showing 1-3 of 886 papers.

Citations

(1) "Biopolymer-based intra-articular delivery of DKK1 into the contracted rabbit knee"
Authors: Suenghwan Jo, et al.
Journal: Gene Reports 2017
Application: Cell-Based Assay
(2) "Neuropeptide Y enhances proliferation and prevents apoptosis in rat bone marrow stromal cells in association with activation of the Wnt/β-catenin pathway in vitro"
Authors: Jianqun Wu, et al.
Journal: Stem Cell Res 2017
Application: Cell Culture
(3) "The effect of C-X-C motif chemokine 13 on hepatocellular carcinoma associates with Wnt signaling"
Authors: Li C, et al.
Journal: Biomed Res Int 2015
Application: Cell Culture