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Recombinant human Annexin A5 Protein, His Tag, 1mg  

Recombinant human Annexin A5 Protein, His Tag, 1mg

Recombinant Human Annexin A5 Protein, Met1-Aps320 produced in E.coli cells, His Tag

Synonym
recombinant human protein, ANXA5, ANX5, ENX2, PP4, annexin V, Annexin A5

More details

AN5-H5123-1K

Availability: within 7 days

1 078,00 €

Background
Annexin A5 is a phospholipid binding protein, which binds with high affinity and selectivity to PS in the presence of calcium. PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Anexin A5 will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Anexin A5 has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Anexin A5 is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). [1,2]

Source
Recombinant Human Annexin A5 Protein (rhANXA5) Met1-Aps320 (Accession #AAH01429 ) was produced in E.coli cells.

Molecular Characterization
rhANXA5 contains C-terminal polyhistidine tag and has a calculated MW of 36.8 kDa. DTT-reduced protein migrates as 36.8 kDa estimated polypeptide in SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg of the rhANXA5 by the LAL method.

Purity
>98% purity as determined by SDS-PAGE of reduced rhANXA5.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

References

(1) Adekunle I. Elegbede a, D.K. Srivastava,et al. 2006, Protein Expression and PuriWcation 50: 157–162.
(1) Bent Brachvogela, Heike Welzel, et al. 2001, Mechanisms of Development 109: 389–393.