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Recombinant human ErbB-2/HER2 Protein, With C-Fc Tag, 1mg  

Recombinant human ErbB-2/HER2 Protein, With C-Fc Tag, 1mg

Recombinant Human Her2, Fc Tag (HE2-H5253) is expressed from human 293 cells (HEK293)

Synonym
recombinant, human, protein, ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN19, NEU, NGL, TKR1, ERBB-2, MLN-19, TKR-1, Epidermal-growth-factor-Receptor-2

More details

HE2-H5253-1K

Availability: within 7 days

1 800,00 €

Background
Human Epidermal growth factor Receptor 2 (HER2),also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation.[1] HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation .[2-6]

Source
Recombinant Human Her2, Fc Tag (HE2-H5253) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
Predicted N-terminus: Thr 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 96.0 kDa. The protein migrates as 125-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhHER2-Fc by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human Her2, Fc Tag (Catalog # HE2-H5253) at 0.05 μg/mL (100 μL/well) can bind Herceptin with a linear range of 0.4-13 ng/mL (QC tested).

References

(1) Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun.208:1067.
(2) Codony-Seeervat, J. et al., 1999, Cancer Res. 59: 1196-1201.
(3) Tzahar, E. et al., 1998, Biochim. Biophys. Acta. 1377: 25-37.
(4) Kermit, L. et al, 1999, J. Biol. Chem. 274: 5263-5266.
(5) Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.