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Recombinant human RENIN Protein,His Tag,  1mg  

Recombinant human RENIN Protein,His Tag, 1mg

Recombinant Human RENIN Protein (rhRenin) Leu24 -Arg406, produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Human Protein, CYMP, CYM, RENNIN, Chymosin

More details

REN-H5221-1K

Availability: within 7 days

1 800,00 €

Background
Renin, also known as REN and angiotensinogenase, is a circulating enzyme that participates in the body\'s renin-angiotensin system (RAS), and plays an essential role in the elevation of arterial blood pressure and increased sodium retention by the kidney. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. Renin is secreted from kidney cells, which are activated via signaling from the macula densa, which responds to the rate of fluid flow through the distal tubule, by decreases in renal perfusion pressure (through stretch receptors in the vascular wall), and by sympathetic nervous stimulation, mainly through beta-1 receptor activation. Renin can bind to ATP6AP2, which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin. In addition, renin binding results in phosphorylation of serine and tyrosine residues of ATP6AP2.[1] The level of renin mRNA appears to be modulated by the binding of HADHB, HuR and CP1 to a regulatory region in the 3\' UTR.[2] An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. These effects lead to hypertension. Therefore, renin inhibitors can be used for the treatment of hypertension.[3]

Source
Recombinant Human RENIN Protein (rhRenin) Leu24 -Arg406 (Accession # AAH33474) was produced in human 293 cells (HEK293).

Molecular Characterization
rhRenin, fused with 6×his tag at the C-terminus and has a calculated MW of 43.2 kDa expressed. The predicted N-terminus is Leu24. Protein migrates as 45 kDa in reduced SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhRenin by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
N/A

References

(1) Nguyen G, et al., 2002, J. Clin. Invest. 109 (11): 1417–27.
(2) Adams DJ, et al., 2003, J. Biol. Chem. 278 (45): 44894–903.
(3) Ram CV., 2009, Future Cardiol 5 (5): 453–65.