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Recombinant human FOLR2 Protein, His Tag, 1mg  

Recombinant human FOLR2 Protein, His Tag, 1mg

Recombinant Human FOLR2 /FBP Protein (rhFOLR2) Thr17-His228 was produced in human 293 cells (HEK293), His Tag

Synonym: recombinant human protein, FOLR2, FOLR-2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, Folate-receptor-beta

More details

FO2-H5223-1K

Availability: within 7 days

1 100,00 €

Background
Folate receptor beta is also known as Folate receptor 2, FBP, FOLR2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, and is a member of the folate receptor (FOLR) family. and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers. It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.

Source
Recombinant Human FOLR2 /FBP Protein (rhFOLR2) Thr17-His228 (Accession # P14207-1) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to N-linked glycosylation which has been verified by PNGase F digestion.

Endotoxin
Less than 1.0 EU per μg of the rhFOLR2 by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. When Folic AcidBovine Serum Albumin was coated at 5 μg/ml (100 μl/well), the concentration of rhFOLR2 that produces 50% of the optimal binding response was found to be approximately 0.15-1.1 nM.


References

(1) Ratnam, M. et al., 1989, Biochemistry, 28(20):8249-54.
(2) Ross, J.F. et al., 1999, Cancer, 85:348
(3) Reddy, J.A. et al., 1999, Blood, 93:3940
(4) Ross, J.F. et al.,1994, Cancer 73:2432
(5) van der Heijden, J.W. et al., 2009, Arthritis Rheum. 60:12
(6) Nagai, T. et al., 2009, Cancer Immunol. Immunother. Feb 24 epub