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Recombinant human FOLR1 Protein, His Tag, 100µg  

Recombinant human FOLR1 Protein, His Tag, 100µg

Recombinant human FOLR1 Protein (rhFOLR1) Arg 25-Met 233, produced in human 293 cells (HEK293), His Tag

Synonym: Recombinant human protein, FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha

More details

FO1-H5229-100

Availability: within 7 days

420,00 €

Background
Folate Receptor 1 (FOLR1) also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage [1-3]. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas [4]. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface [3, 5]. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk [6]. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.[7]

Source
Human FOLR1, His Tag (FO1-H5229) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).
Predicted N-terminus: Arg 25

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.4 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhFOLR1 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Refer to data sheet

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Human FOLR1, His Tag (Cat. No. FO1-H5229) ELISA bioactivity
Immobilized Folic Acid-BSA Conjugate at 20 μg/mL (100 μL/well) can bind Human FOLR1, His Tag (Cat. No. FO1-H5229) with a linear range of 78-625 ng/mL (QC tested).

References

(1) Lacey SW, et al., 1989, J. Clin. Invest. 84 (2): 715–20.
(2) Elwood PC, 1989, J. Biol. Chem. 264 (25): 14893–901.
(3) Rijnboutt, S. et al., 1996, J. Cell Biol. 132:35.
(4) Parker, N. et al., 2005, Anal. Biochem. 338:284.
(5) Paulos C M, et al., 2004, Mol Pharmacol. 2004;66:1406–1414.
(6) Elwood, P.C. et al., 1991, J. Biol. Chem. 26:2346.
(7) Dainty,L.A. et al., 2007, Gynecol Oncol. 105 (3):563-70.