Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human BACE1 Protein, Tag Free (active enzyme, MALS verified), 1mg  

Recombinant human BACE1 Protein, Tag Free (active enzyme, MALS verified), 1mg

Recombinant Human BACE1 Protein, Native (rhBACE1) Thr22-Thr 457, produced in human 293 cells (HEK293), Tag Free (active enzyme, MALS verified)

Synonym: recombinant, human, protein BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Memapsin-2

More details

BA1-H5213-1K

Availability: within 7 days

1 800,00 €

Background
Beta-secretase 1 (BACE1), also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer\'s disease, and in the formation of myelin sheaths in peripheral nerve cells.[1] The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer\'s patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. [2-3]

Source
Recombinant Human BACE1 Protein, Native (rhBACE1), Tag Free (BA1-H5213) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Thr 457 (Accession # NP_036236.1).
Predicted N-terminus: Thr 22

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 49.0 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhBACE1 by the LAL method.

Purity
>98% purity as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Rotational Variance-Based Data Augmentation in 3D Graph Convolutional Network"
Kim, Kim, Lee et al
Chem Asian J (2021)
(2) "Identification of plant-based multitargeted leads for Alzheimer's disease: In-vitro and in-vivo validation of Woodfordia fruticosa (L.) Kurz"
Raghuvanshi, Nuthakki, Singh et al
Phytomedicine (2021) 91, 153659
(3) "Evaluation of putative CSF biomarkers in paediatric spinal muscular atrophy (SMA) patients before and during treatment with nusinersen"
Johannsen, Weiss, Daubmann et al
J Cell Mol Med (2021)
Showing 1-3 of 580 papers.
Please refer to product data sheet.