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Recombinant human CADM1, 1mg  

Recombinant human CADM1, 1mg

Recombinant Human CADM1 / IGSF4A Protein (rhCADM1), Gln45-His374 expressed in human 293 cells (HEK293), His Tag.

Synonym: recombinant, human, protein CADM1; CADM-1;BL2; Cell Adhesion Molecule 1

More details

CA1-H5225-1K

Availability: within 7 days

1 800,00 €

Background
Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. Cell adhesion molecule 1 is a single-pass type I membrane protein which belongs to the nectin family. Cell Adhesion Molecule 1 contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain, and is also known as CADM1; BL2; DKFZp686F1789; IGSF4; IGSF4A; MGC149785; MGC51880; NECL2; Necl-2; RA175; ST17; SYNCAM; TSLC1; sTSLC-1; sgIGSF; synCAM1. CADM1 mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca2+-independent manner. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. CADM1 may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.[1-5].

Source
Recombinant Human CADM1 Protein (rhCADM1), Gln45-His374 (Accession# NP_055148.3)was expressed in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.9 kDa. The protein migrates as 50 kDa and 55-85 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCADM1 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE of reduced rhCADM1.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity-ELISA
Immobilized Cynomolgus CRTAM, Fc Tag (Cat. No. CRM-C5255) at 5 μg/mL (100 μL/well) can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with a linear range of 0.6-5 ng/mL (QC tested).

Bioactivity-BLI
Loaded Human CRTAM, His Tag (Cat. No. CRM-H52H3) on AR2G Biosensor, can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with an affinity constant of 51.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Cynomolgus CRTAM, Fc Tag (Cat. No. CRM-C5255) on Protein A Biosensor, can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with an affinity constant of 37.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

References

(1) Kuramochi M. 2001, Nat. Genet. 27: 427-430.
(2) Yageta M. et al., 2002, Cancer Res. 62: 5129-5133.
(3) Furuno T. et al., 2005, J. Immunol. 174: 6934-6942.
(4) Boles KS. et al., 2005, Blood. 106: 779-786.
(5) Dong X. et al., 2006  J. Biol. Chem. 281: 10610-7.