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Recombinant human IL13RA1 /CD213A1 Protein, His Tag, 1mg  

Recombinant human IL13RA1 /CD213A1 Protein, His Tag, 1mg

Recombinant Human IL13RA1 Protein, Gly22-Thr343 was produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Human Protein, IL13RA1, CD213A1, IL-13Ra, NR4, RP13-128O4.2, IL13Rα1

More details

IL1-H5224-1K

Availability: within 7 days

1 800,00 €

Background
Interleukin 13 receptor, alpha 1, also known as IL13RA1, NR4 and CD213A1 (cluster of differentiation 213A1), The IL13 Rα1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL13Rα1 share 76% aa sequence identity. IL13RA1 is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.[1-3]

Source
Recombinant Human IL13RA1 Protein, (rhIL13RA1) Gly22-Thr343 (Accession # NP_001551.1)was produced in human 293 cells (HEK293).

Molecular Characterization
rhIL13RA1, fused with a 6×his tag at the C-terminus and the protein has a calculated MW of 38 kDa. DTT-reduced protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL13RA1 by the LAL method.

Purity
>98% purity as determined by SDS-PAGE of reduced rhIL13RA1.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C). 

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human IL-13RA1, His Tag (Catalog # IL1-H5224) at 10μg/mL (100 μL/well) can bind Human IL-13, Fc Tag (Catalog # IL3-H4269) with a linear range of 0.64-10 μg/mL.

References

(1) Caput, D. et al., 1996, J. Biol. Chem. 271:16921.
(2) Donaldson, D.D. et al., 1998, J. Immunol. 161:2317.
(3) Hilton, D.J. et al., 1996, Proc. Natl. Acad. Sci. USA 93:497.