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Recombinant human CEACAM6 /CD66c Protein, 1mg  

Recombinant human CEACAM6 /CD66c Protein, 1mg

Recombinant Human CEACAM6 /CD66c Protein (rhCEACAM6) Lys35-Gly320 was produced in human 293 cells (HEK293).

Synonym: recombinant, human, protein CEACAM6,CD66c,CEAL,NCA 

More details

CE6-H5223-1K

Availability: within 7 days

1 500,00 €

Background
Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression. [1-5]

Source
Recombinant Human CEACAM6 /CD66c Protein (rhCEACAM6) Lys35-Gly320 (Accession # NP_002474.3)was produced in human 293 cells (HEK293).

Molecular Characterization
rhCEACAM6, fused with 6×his tag at C-terminus and has a calculated MW of 32 kDa expressed. The predicted N-terminus is Lys35. Protein migrates as 35-65 kDa in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCEACAM6 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
N/A

References

(1) Beauchemin, N. et al., 1999, Exp. Cell Res. 252:243.
(2) Skubitz, K.M. et al., 1999, J. Biol. Regul. Homeost. Agents 13:244.
(3) Tawaragi, Y. et al.,1988, Biochem. Biophys. Res. Comm. 150:89.
(4) Scholzel, S. et al., 2000, Am. J. Pathol. 156: 595-605.
(5) Duxbury, M.S. et al., 2004, J. Biol. Chem. 279: 23176-23182.