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Recombinant human CTLA4/CD152 Protein, His Tag, 1mg  

Recombinant human CTLA4/CD152 Protein, His Tag, 1mg

Recombinant Human CTLA4 /CD152 Protein (rhCTLA4) Ala 37 - Phe 162, produced in human 293 cells (HEK293), His Tag

Synonym
rembinant human protein CTLA4, CD152

More details

CT4-H5229-1K

Availability: within 7 days

935,00 €

Background
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Source
Recombinant Human CTLA4 /CD152 Protein (rhCTLA4) Ala 37 - Phe 162 (Accession # NP_005205.2) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.4 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCTLA4 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human B7-2, Fc Tag (Catalog # CD6-H5257) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Catalog # CT4-H5229) with a linear range of 1-6.4 ng/mL.
Immobilized Human B7-1, Fc Tag (Catalog # B71-H5259) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Catalog # CT4-H5229) with a linear range of 0.16-2.56 ng/mL.


References

(1) Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
(2) Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
(3) Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.