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Recombinant human IL6R Protein, His Tag, 50µg  

Recombinant human IL6R Protein, His Tag, 50µg

Recombinant Human IL6R Protein Leu20-Pro365, produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein, IL6R, CD126, IL-6R-1, IL-6RA, IL6RA, IL-6R-alpha, IL6RQ, gp80

More details

ILR-H4223-050

Availability: within 7 days

378,00 €

Background
Interleukin 6 receptor (IL6R) also known as CD126 (Cluster of Differentiation 126) , is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL6R is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit also shared by many other cytokines. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor.[1-4]

Source
Recombinant Human IL6R Protein (rhIL6R) Leu20-Pro365 (Accession # NP_000556.1)was produced in human 293 cells (HEK293).

Molecular Characterization
rhIL6R, fused with 6×his tag at the N-terminus, has a calculated MW of 40 kDa expressed. The predicted N-terminus is Leu20. Protein migrates as 60-70 kDa in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL6R by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human IL-6, Tag Free (Catalog # IL6-H4218) at 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha, His Tag (Catalog # ILR-H4223) with a linear range of 1-16 ng/mL (QC tested).

References

(1) Taga, T., et al., 1989, Cell (UNITED STATES) 58 (3): 573–81.
(2) Schooltink, H., 1992, FEBS Lett. (NETHERLANDS) 314 (3): 280–4.
(3) Yoshio-Hoshino, N. et al., 2007, Cancer. Res. 67: 871-875.
(4) Egler, R.A. et al., 2008, Clin. Cancer. Res. 14: 7028-7034.