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Recombinant human GFRA1 /GDNFRA Protein, His Tag, 4x250ug  

Recombinant human GFRA1 /GDNFRA Protein, His Tag, 4x250ug

Recombinant Human GFR alpha-1 Protein (Human GFR alpha-1, His Tag) Asp 25 - Ser 424 was produced in human 293 cells (HEK293)

Synonym
recombinant, human protein, GFRA1, GDNFRA, RETL1, TRNR1, GDNFR, GFR-ALPHA-1, RET1L

More details

GF1-H5224-1K

Availability: within 7 days

1 600,00 €

Background
GDNF family receptor alpha-1 (GFRA1) is also known as RET ligand 1 (RETL1), TGF-beta-related neurotrophic factor receptor 1 (TRNR1), is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell line-derived neurotrophic factor (GDNF) receptor subfamily. The GDNF family ligands comprises GDNF, Neurturin (NTN), Artemin, and Persephin. GDNF and NTN are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFRA1 mediates the association with and activation of the RET tyrosine kinase receptor (RTK), and subsequently initiates the RET signaling pathway. GFR alpha-mediated signaling plays an important role in the survival, differentiation, and migration of central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system, as well as the inflammatory response in some carciniomas.

Source
Recombinant Human GFR alpha-1 Protein (Human GFR alpha-1, His Tag) Asp 25 - Ser 424 (Accession # NP_665736.1) was produced in human 293 cells (HEK293).

Molecular Characterization
rhGFRA1, fused with 6×His tag at the C-terminus, has a calculated MW of 45.5 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhGFRA1 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF at 1 µg/mL binds rhGFRA1 with an apparent KD <10 nM.

References

(1) Angrist M, et al., 1998, Genomics 48 (3): 354–62.
(2) Jing, S., et al., 1997, J. Biol. Chem. (UNITED STATES) 272 (52): 33111–7.
(3) Cik, M., et al., 2000, J. Biol. Chem. (UNITED STATES) 275 (36): 27505–12.
(4) Klein, R D., et al., 1997, Nature (ENGLAND) 387 (6634): 717–21.