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Recombinant human GFRA2 /GDNFRB Protein, His Tag, 1mg  

Recombinant human GFRA2 /GDNFRB Protein, His Tag, 1mg

Recombinant Human GFRA2 /GDNFRB Protein (rhGFRA2) Ser 22 - Ser 441 was produced in human 293 cells (HEK293) His Tag

Synonym
recombinant human protein, GFRA2, GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2

More details

GF2-H5225-1K

Availability: within 7 days

1 200,00 €

Background
GDNF family receptor alpha-2 (GFRA2) is also known as GDNF receptor beta, Neurturin receptor alpha, RET ligand 2, TGF-beta-related neurotrophic factor receptor 2, is a cell membrane protein which belongs to the GDNFR family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF-beta superfamily of growth factors. Three receptors for these factors, GFRα-1, GFRα-2 and GFRα-3 have been identified. The isoform 1 of GFRA2 is found in both brain and placenta. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor and also able to mediate GDNF signaling through the RET tyrosine kinase receptor. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It can also mediate GDNF signaling through the RET tyrosine kinase receptor.

Source
Recombinant Human GFRA2 /GDNFRB Protein (rhGFRA2) Ser 22 - Ser 441 (Accession # NP_001486.4) was produced in human 293 cells (HEK293).

Molecular Characterization
rhGFRA2, fused with 6×His tag at the N-terminus, has a calculated MW of 47.6 kDa. The predicted N-terminus is Ser 22. DTT-reduced Protein migrates as 60-70 KDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhGFRA2 by the LAL method.

Purity
>97% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neurturin at 1 µg/ml can bind rhGFRA2 with an apparent KD < 8 nM.

References

(1)Sanicola M., et al., 1997, Proc Natl Acad Sci U S A 94 (12): 6238–6243.
(2)Hisasue S.,et al., 2006, Neurobiol Aging. 27 (10): 1524-1530.
(3)Souza RP., et al., 2010, Psychopharmacology. 210 (3): 347-354.