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Recombinant human IL1R2/CD121b Protein, Fc Tag, 100µg  

Recombinant human IL1R2/CD121b Protein, Fc Tag, 100µg

Recombinant Human IL1R2 /CD121b Protein, Thr 14 - Glu 343 was produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein, CD121b, IL1RB, IL1R2, CDw121b

More details

IL2-H4256-100

Availability: within 7 days

312,00 €

Background
Interleukin-1 receptor type 2 (IL1R2) also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

Source
Human IL-1 RII, Fc Tag (IL2-H4256) is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1). Predicted N-terminus: Phe 14

Molecular Characterization
rh IL1R2 /CD121b, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 64 kDa. The predicted N-terminus is Thr 14. DTT-reduced Protein migrates as 68-74 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh IL1R2 /CD121b by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B protein (Catalog # ILB-H4110) at 2 μg/ml can bind rh IL1R2 /CD121b Fc Chimera with a linear range of 6.25 - 500 ng/ml.

References

(1) Liu C., et al., 1996, J. Biol. Chem. 271:20965-20972.
(2) Giri J.G., et al., 1994, J. Immunol. 153:5802-5809.
(3) Symons J.A., et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92:1714-1718.
(4) Orlando S., et al., 1997, J. Biol. Chem. 272:31764-31769.
(5) Wang D., et al., 2010, Nat. Immunol. 11:905-911.